There is relatively little information avialable regarding the role of plant growth regulators in the germination of recalcitrant seeds. Pieruzzi et al. determine an increase in the ratio of the polyamines (spermidine + spermine) : putrescine in the embryos of germinating Araucaria angustifolia (gymnosperm) and Ocotea odorifera (angiosperm), which could be used as a marker for germination completion. An unexpected increase of ABA levels was observed in A. angustifolia embryos, which suggests a potential role for this rgeulator in some recalcitrant seeds.
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