Secondary somatic embryogenesis has been postulated to occur during induction of peach palm (Bactris gasipaes) somatic embryogenesis. Steinmacher et al. study this morphogenetic pathway and use zygotic embryos as explants to develop a technique for the establishment of cycling cultures using a temporary immersion system (TIS). They confirm the occurrence of secondary somatic embryos in peach palm and describe a feasible protocol for regeneration in vitro. Plantlets are obtained and after 3 months in culture their growth is significantly better in TIS than on solid culture medium.
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