Dynamic processes occurring at the soil–root interface crucially influence soil physical, chemical and biological properties at a local scale around the roots, and are technically challenging to capture in situ. Rudolph-Mohr et al. introduce a novel combination of neutron and fluorescence imaging to study the spatiotemporal patterns of oxygen, pH and water content distribution in the rhizosphere of living roots of lupin (Lupinus albus) grown in rhizotrons. The combined imaging set-up enables them to relate observed changes in soil pH and oxygen levels to root structure and soil water content non-destructively.
Combining neutron and fluorescence imaging allows study of the spatiotemporal patterns of oxygen, pH and water content distribution in lupin rhizospheres.