Tissue-specific expression of NPC2 by histochemical GUS reporter assay

Non-Specific Phospholipase C2 (NPC2) in plant immune responses in Arabidopsis

The non-specific phospholipases C (NPC) are recently discovered new subclass of plant phospholipases. Most of them have not yet been characterised thoroughly.

Tissue-specific expression of NPC2 by histochemical GUS reporter assay
Tissue-specific expression of NPC2 by histochemical GUS reporter assay: (A) 7-d-old seedling, (B) 10-d-old seedling, (C) 14-d-old seedling, (D) inflorescence, (E) flower, (F) siliques, (G) young leaf, (H) old leaf (D–H, 5-week-old plants), (I) main root (14-d-old seedling), (J) lateral root (10-d-old seedling). Scale bars: A–C, E 1 mm; D, F, G, H 2 mm; I, J 0.1 mm.

Krčková et al. prepare and purify Arabidopsis thaliana NPC2 in a heterologous system, and experimentally proved its phospholipase activity in vitro. Using lines overexpressing NPC2: GFP. They determine its endomembrane (preferentially Golgi apparatus) localisation. NPC2 transcription is strongly downregulated under pathogen attack or by treatment with elicitors or bacterial effectors. Furthermore, when treated with an elicitor, lines constitutively overexpressing NPC2 produce higher level of reactive oxygen species – a hallmark of plant immune reaction. These findings imply NPC2 function in plant immunity.


The Annals of Botany Office is based at the University of Oxford.

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